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| Id: | 27148
| | Autor: | Silva, Manuel T; Portaels, Françoise; Macedo, Paula M. | | Título: | New data onthe ultrastructure of the membrane of Mycobacterium leprae.
| | Fonte: | Int J Lep;57(1):54-64, Mar. 1989. ^bilus, ^btab, ^bgraf.
| | Resumo: | In previous reports on the ultrastructure of Mycobacterium leprae, we described the occurrence of symmetric membranes in normal-looking bacilli from fresh or frozen samples primarily fixed with aldehydes. In those reports we admitted that such a symmetric profile, which is not found in the other normal mycobacteria, would not represent the structure of the normal membrane of the leprosy bacillus. We, therefore, re-analyzed the ultrastructure of the membrane of M. leprae. In the present work the micromorphology of the M. leprae membrane was studied by transmission electronmicroscopy after the fixation of fresh samples by OsO4 plus calcium followed by glutaraldehyde plus formaldehyde and calcium followed by uranyl acetate. The study of samples from two patients with lepromatous (LL) leprosy, three armadillos with natural leprosy, and one nude mouse with experimental leprosy showed that normal-looking bacilli present in lead-stained sections had asymmetric membranes with a thickness of 6.49 +/- 0.36 nm. These membranes showed periodic acid-Schiff (PAS)-positive components exclusively located in the outer half of the bilayer. We demonstrated that the symmetric profile of the M. leprae membrane described in our previous reports corresponds, as admitted in those reports, to an abnormal membrane structure. Such an abnormality was now found to result from the use of primary fixation with aldehydes or of samples stored frozen before fixation. These results indicate that, although ultrastructurally similar to that of the other mycobacteria, the membrane of M. leprae has a peculiar sensitivity to fixation by aldehydes. Such a characteristic, which was not found in M. lepraemurium, M. aurum, M. avium, and M. tuberculosis H37Ra, must reflect a unique membrane molecular structure, which is presently unknown^ien.
| | Descritores: | Mycobacterium leprae/ultraest
Mycobacterium avium/ultraest
Membrana Celular/ultraest
| | Limites: | Animais
| | Meio Eletrônico: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n1/v57n1a08.pdf - en.
| | Localização: | Br191.1 |
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